human osteocalcin duoset elisa kit  (R&D Systems)

Journal: Journal of Functional Biomaterials

Article Title: Impact of Vitamin D 3 Functionalization on the Osteogenic Capacity of Bioinspired 3D Scaffolds Based on Ce-Doped Bioactive Glass and Spongia Agaricina

doi: 10.3390/jfb16040141

Figure Lengend Snippet: Micrographs of DPSC cultivated in osteogenic medium for 21 days in the presence of S3Ce and S3CeD3 scaffolds showing ( a ) mineralization nodules stained with Alizarin Red S and ( b ) quantitative data based on absorbance at 562 nm. * p < 0.05 compared to control, # p < 0.05 compared to S3Ce. Scale bar = 100 µm. Calcium ( c ) and osteocalcin ( d ) concentrations secreted by cells osteogenically differentiated in the presence of non-functionalized scaffolds for 14 and 21 days, estimated with a colorimetric kit and ELISA kit, respectively. Negative control: cells cultivated only in osteogenic medium. Values are expressed as the mean ± SD ( n = 9), * p < 0.05 compared to control, # p < 0.05 compared to S3Ce.

Article Snippet: The osteocalcin concentration was measured using an ELISA kit (R&D Systems, Minneapolis, MN, USA).

Techniques: Staining, Control, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: IEEE Open Journal of Nanotechnology

Article Title: Pulsed Electromagnetic Field-Assisting Reduced Graphene Oxide-Incorporated Nanofibers for Osteogenic Differentiation of Human Dental Pulp Stem Cells

doi: 10.1109/ojnano.2024.3494770

Figure Lengend Snippet: FIGURE 5. Osteogenic differentiation study of RGO-NFs under PEMF conditions. (a) Cell viability assay under PEMF conditions. AF-w/PEMF samples enhanced cell viability, with AF0.1%-w/PEMF recording the highest cell viability by day 7. (b) ALP activity assay in week 1. AF0.1%-w/PEMF exhibited significantly higher ALP secretion, indicating enhanced early-stage osteogenic differentiation. (c) ARS staining of DPSCs cultured on AFs under PEMF conditions. AF-w/PEMF application enhanced calcium deposition across all RGO concentrations. (d) Quantitative analysis of ARS results. AF0.1%-w/PEMF showed significantly higher calcium deposition compared to AF-w/oPEMF samples. (e) ELISA results for OCN at week 2. AF0.1%-w/PEMF showed the highest OCN expression, although differences were not statistically significant. (f) Fluorescence microscopy images of DPSCs under osteogenic differentiation conditions with PEMF stimulation. AF0.1%-w/PEMF exhibited the highest OCN expression. Error bars in A, B, D and E represent mean ± standard deviation (n = 5). Statistical analysis was performed using the LSD test for A, B, D and E (p < 0.05). Same letters indicate no significant differences between groups, while different letters indicate significant differences between groups. Groups marked with combined letters are not significantly different from groups marked with either of those letters. DPSCs, dental pulp stem cells; AFs, aligned fibers; PEMF, pulsed electromagnetic field; OCN, osteocalcin; ALP, alkaline phosphatase; ELISA, enzyme-linked immunosorbent assay; ARS, alizarin red staining; RGO, reduced graphene oxide; LSD, least significant difference.

Article Snippet: All analytical chemicals, media components, and reagents used included the following substances: PCL (440744, Sigma-Aldrich, St. Louis, MO, USA), chloroform (C0588, SAMCHUN, Pyeongtaek, Republic of Korea), N,N-dimethylformamide (DMF; 3057-4105, DAEJUNG, Siheung, Republic of Korea), fetal bovine serum (FBS; S001-07, Welgene, Gyeongsan, Republic of Korea), L-ascorbic acid (A4544, Sigma-Aldrich, St. Louis, MO, USA), antibiotics (LS203-01, Welgene, Gyeongsan, Republic of Korea), sodium bicarbonate (2736, DUKSAN, Ansan, Republic of Korea), Trypsin-EDTA solution (LS 015-10, Welgene, Gyeongsan, Republic of Korea), minimum essential medium (MEM; M4655, Sigma-Aldrich, St. Louis, MO, USA), dexamethasone (D4902, Sigma-Aldrich, St. Louis, VOLUME 5, 2024 125 LEE ET AL.: PEMF-ASSISTING RGO-INCORPORATED NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION MO, USA), β-glycerophosphate (G9422, Sigma-Aldrich, St. Louis, MO, USA), Water-Soluble Tetrazolium-1 (WST-1) assay kit (EZ-3000, Daeillab, Seoul, Republic of Korea), phosphate- buffered saline (PBS; P7059, Sigma-Aldrich, St. Louis, MO, USA), SensoLyte ALP Assay kit (AS-72146, AnaSpec, Fremont, CA, USA), BCA protein assay kit (23225, Thermo Fisher Scientific, Rockford, IL, USA), enzyme-linked immunosorbent assay (ELISA) kit (DY1419-05, R&D Systems, Minneapolis, MN, USA ), 4% paraformaldehyde solution (P6148, Sigma-Aldrich, St. Louis, MO, USA), ARS (A5533, Sigma-Aldrich, St. Louis, MO, USA), cetylpyridinium chloride (C0732, Sigma-Aldrich, St. Louis, MO, USA), Triton X-100 (X-100, Sigma-Aldrich, St. Louis, MO, USA), TRITC-conjugated phalloidin (FAK100, Millipore, Billerica, MA, USA), anti-osteocalcin (anti-OCN; SAB5702281, Sigma-Aldrich, St. Louis, MO, USA), anti-Cx43 (SAB4501174, Sigma-Aldrich, St. Louis, MO, USA), and 4’,6-diamidino-2-phenylindole (DAPI; FAK100, Millipore, Billerica, MA, USA).

Techniques: Viability Assay, ALP Activity Assay, Staining, Cell Culture, Enzyme-linked Immunosorbent Assay, Expressing, Fluorescence, Microscopy, Standard Deviation

Journal: Biomaterials advances

Article Title: Impact of degradable magnesium implants on osteocytes in single and triple cultures.

doi: 10.1016/j.msec.2022.112692

Figure Lengend Snippet: Fig. 4. A-E Gene expression of osteocyte markers osteocalcin (BGLAP), E11, PHEX and RANKL, as well as expression of the osteoblast marker ALPL were analyzed for osteocytes, differentiated from osteoblasts of three donors (each n = 3, n = 9 in total, see Table 1). Diagrams show fold changes compared to the control (without contact to Mg discs) ± upper and lower limit, F: osteocalcin expression was quantified on protein level using ELISA. Diagram shows averages ± standard deviation (n = 9). One way ANOVA followed by Tukey post-hoc test, *p < 0.05, ** p < 0.01 compared to the control.

Article Snippet: A solid phase sandwich ELISA kit (Human Osteocalcin DuoSet ELISA # DY1419–05; R&D Systems, USA) was used for analyzing osteocalcin content.

Techniques: Gene Expression, Expressing, Marker, Control, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: Biomaterials advances

Article Title: Impact of degradable magnesium implants on osteocytes in single and triple cultures.

doi: 10.1016/j.msec.2022.112692

Figure Lengend Snippet: Fig. 6. A–F Gene expression of osteocyte markers RANKL, PHEX, MEPE, E11 and BGLAP (osteocalcin), and of the osteoblast marker ALPL was analyzed in five independent experiments involving osteoblasts and osteocytes of three donors and osteoclasts of four donors, each n = 3, n = 15 in total (see Table 1). Diagrams show fold changes compared to the control (without Mg), ±upper and lower limit, * p < 0.05, ***p < 0.001. High upper and lower limits occur due to the combination of the five different experiments due to variations between the different donors. Therefore, gene expression for the single experiments is shown in Fig. S1. F: Osteocalcin concentration in the supernatant of the cultures was analyzed by ELISA for two independent experiments. Data of the single experiments are shown in Fig. S1. Average ± standard deviation (n = 6).

Article Snippet: A solid phase sandwich ELISA kit (Human Osteocalcin DuoSet ELISA # DY1419–05; R&D Systems, USA) was used for analyzing osteocalcin content.

Techniques: Gene Expression, Marker, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

Journal: International Journal of Environmental Research and Public Health

Article Title: The Impact of Nordic Walking on Bone Properties in Postmenopausal Women with Pre-Diabetes and Non-Alcohol Fatty Liver Disease

doi: 10.3390/ijerph18147570

Figure Lengend Snippet: Baseline characteristics of the exercise and control group.

Article Snippet: The serum concentration of OC as a bone formation marker was assessed by ELISA using Human Osteocalcin Quantikine ELISA kit (produced by R&D Systems, Minneapolis, MN, USA; assay sensitivity 0.898 ng/mL).

Techniques: Control, Activity Assay